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Publication Detail
Electrically sensing protease activity with nanopores
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Kukwikila M, Howorka S
  • Publisher:
    IOP PUBLISHING LTD
  • Publication date:
    17/11/2010
  • Journal:
    J PHYS-CONDENS MAT
  • Volume:
    22
  • Issue:
    45
  • Print ISSN:
    0953-8984
  • Language:
    EN
  • Keywords:
    DNA ANALYSIS, ALPHA-HEMOLYSIN, PEPTIDES, PORE, IDENTIFICATION, COMPLEXES, MOLECULES, TRANSPORT, MEMBRANE, CHANNELS
Abstract
The enzymatic activity of a protease was electrically detected using nanopore recordings. A peptide substrate was tethered to microscale beads, and cleavage by the enzyme trypsin released a soluble fragment that was electrophoretically driven through the alpha-hemolysin protein pore, leading to detectable blockades in the ionic current. Owing to its simplicity, this approach to sense enzymatic activity may be applied to other proteases.
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