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Publication Detail
Development of a novel multi-triplex qPCR method for the assessment of bacterial community structure in oral populations
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Ciric L, Pratten J, Wilson M, Spratt D
  • Publisher:
    WILEY-BLACKWELL PUBLISHING, INC
  • Publication date:
    12/2010
  • Pagination:
    770, 774
  • Journal:
    ENV MICROBIOL REP
  • Volume:
    2
  • Issue:
    6
  • Print ISSN:
    1758-2229
  • Language:
    EN
  • Keywords:
    REAL-TIME PCR, PORPHYROMONAS-GINGIVALIS, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, EXPERIMENTAL GINGIVITIS, SUBGINGIVAL PLAQUE, MOLECULAR ANALYSIS, QUANTITATIVE PCR, CULTURE METHODS, HEALTH, CARIES
  • Addresses:
    Ciric, L
    UCL Eastman Dent Inst
    Dept Microbial Dis
    London
    WC1X 8LD
    England
Abstract
P>Gingivitis and dental caries are two of the most predominant diseases in humans. Both conditions are easily treated with the removal of the plaque biofilm by brushing or the use of oral hygiene products. In both cases, pathogenic taxa found within the plaque biofilm are the causal agents of the disease. Actinomyces naeslundii, Fusobacterium nucleatum and Prevotalla intermedia have all been implicated in the development of gingivitis, while Streptococcus mutans is the main organism associated with dental caries. Many studies have so far focused on the use of culture methods to detect and enumerate the pathogenic taxa within plaque samples. However, these methods are both labour intensive and biased towards culturable taxa. In the present study, a novel high-throughput multi-triplex quantitative PCR method was developed with the aim to investigate the community dynamics associated with oral communities. Three triplex assays were designed targeting taxa associated with gingivitis and dental caries as well as oral health. Saliva samples collected from healthy individuals were used in order to validate the newly developed method.
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Dept of Civil, Environ & Geomatic Eng
Eastman Dental Institute
Microbial Diseases
Microbial Diseases
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

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