Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at http://www.ucl.ac.uk/finance/research/post_award/post_award_contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Crumbs/DaPKC-dependent apical exclusion of Bazooka promotes photoreceptor polarity remodeling.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Walther RF, Pichaud F
  • Publication date:
  • Pagination:
    1065, 1074
  • Journal:
    Curr Biol
  • Volume:
  • Issue:
  • Status:
  • Country:
  • PII:
  • Language:
  • Keywords:
    Animals, Cell Polarity, Drosophila, Drosophila Proteins, Intracellular Signaling Peptides and Proteins, Phosphorylation, Photoreceptor Cells, Invertebrate, Protein Kinase C
BACKGROUND: In Drosophila epithelial cells, specification and maintenance of the zonula adherens (za) is crucial to ensure epithelial tissue integrity. This depends on the intertwined function of Bazooka (Baz), Par6-DaPKC, and the Crumbs (Crb)-Stardust (Sdt)-PATJ complex. However, the detailed molecular basis for the interplay between these factors during this process is not fully understood. RESULTS: We demonstrate that during photoreceptor apicobasal polarity remodeling, Crb is required to exclude Baz from the subapical domain. This is achieved by recruiting Par6 and DaPKC to this membrane domain. This molecular sorting depends on Baz phosphorylation by DaPKC at the conserved serine 980 and on the activity of the small GTPase Cdc42 associated with Par6. Our data indicate that although Cdc42 binding to Par6 is not required for Baz phosphorylation by DaPKC, it is required for optimum recruitment of Crb at the subapical membrane, a process necessary for delineating the nascent za from this membrane domain. CONCLUSION: Binding of Cdc42 to the DaPKC regulatory subunit Par6 is required to promote Crb- and DaPKC-dependent apical exclusion of Baz. This molecular sorting mechanism results in setting up the boundary between the photoreceptor subapical membrane and the za.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Lab for Molecular Cell Bio MRC-UCL
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by