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Publication Detail
Sequence of a novel mRNA coding for a C-terminal-truncated form of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Comparative Study
  • Authors:
    Pichaud F, Frendo JL, Delage-Mourroux R, de Vernejoul MC, Moukhtar MS, Jullienne A
  • Publication date:
    11/09/1995
  • Pagination:
    319, 322
  • Journal:
    Gene
  • Volume:
    162
  • Issue:
    2
  • Status:
    Published
  • Country:
    Netherlands
  • Print ISSN:
    0378-1119
  • PII:
    0378111995003192
  • Language:
    eng
  • Keywords:
    Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary, Gene Expression, HL-60 Cells, Humans, Hydroxyprostaglandin Dehydrogenases, Isoenzymes, Molecular Sequence Data, RNA, Messenger, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid
Abstract
We amplified, using the polymerase chain reaction (PCR) and NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (type-I 15-PGDH)-specific primers, RNA extracted from the HL-60 cell line. Two bands, differing in size by approx. 160 bp, were detected with ethidium bromide staining after electrophoresis of amplification products and hybridization with a 15-PGDH-specific probe. Sequencing these DNA bands revealed that the largest corresponded to the 15-PGDH cloned from human placenta [Ensor et al., J. Biol. Chem. 265 (1990) 14888-14891]. The smaller sequence coded for a predicted C-terminal-truncated form of 15-PGDH. This subtype of the type-I 15-PGDH mRNA was also found using RT-PCR in human liver, placenta and a cell line derived from a human medullary thyroid carcinoma (TT cells). Hybridization studies using specific probes indicated that this new mRNA form probably corresponded to the 3.4-kb mRNA, one of the two 15-PGDH mRNAs previously detected in Northern blot analysis.
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