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Publication Detail
The influence of a bisphosphonate on bone generation determined using a chick-femur model.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Wei LL, Gellynck K, Ng YL, Gulabivala K, Buxton P
  • Publication date:
    06/2011
  • Pagination:
    550, 559
  • Journal:
    Int Endod J
  • Volume:
    44
  • Issue:
    6
  • Status:
    Published
  • Country:
    England
  • Language:
    ENG
  • Keywords:
    Administration, Topical, Animals, Bone Density Conservation Agents, Chick Embryo, Coloring Agents, Diphosphonates, Drug Carriers, Femur, Hydroxyapatites, Organ Culture Techniques, Osteogenesis, Random Allocation, Triazines
Abstract
AIM: To determine the direct influence of a bisphosphonate (pamidronate) delivered by one of two carriers, on bone generation in chick-femurs supported by chick egg chorio-allantoic membranes. METHODOLOGY: Twenty chick femurs freshly harvested from fertilized eggs were randomly allocated to two groups: (i) Affi-Gel blue bead carrier (n=10); and (ii) hydroxyapatite bead carrier (n=10). The femurs in each group were further randomly divided into control (n=4) and experimental (n=6) subgroups. Carriers charged with PBS solution or 0.1 M pamidronate were delivered into the bone marrow of each femur at its mid-portion through a needle puncture. Each femur was then grafted onto the chorio-allantoic membrane of a chick egg and incubated for 7 days. Each experimental and control subgroup femur yielded four histological sections at the puncture site, constituting the test and inter-bone controls. In addition, two histological sections were also obtained from 400 to 450 μm away from each end of the experimental puncture site to act as the intra-bone controls. Bone generation was quantified and the ratio of cross-sectional area of bone marrow to circumference of bone (outcome measure) was determined using a software package, Image-Pro(®) Plus. The data were analysed using Mann-Whitney tests and Wilcoxon signed rank tests. RESULTS: The outcome ratio in the test group was significantly (P<0.001) smaller than both the inter-bone and intra-bone control groups. There was evidence of increased bone formation directly over the pamidronate-charged carriers. CONCLUSIONS: The test model established that pamidronate had a positive effect on bone generation over a period of 7 days, regardless of the carrier type.
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