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Publication Detail
Comparative RNAi screening identifies a conserved core metazoan actinome by phenotype.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Comparative Study
  • Authors:
    Rohn JL, Sims D, Liu T, Fedorova M, Schöck F, Dopie J, Vartiainen MK, Kiger AA, Perrimon N, Baum B
  • Publication date:
  • Pagination:
    789, 805
  • Journal:
    J Cell Biol
  • Volume:
  • Issue:
  • Country:
    United States
  • PII:
  • Language:
  • Keywords:
    Actin Cytoskeleton, Actins, Animals, Carboxy-Lyases, Carrier Proteins, Cell Line, Cell Nucleus, Cell Shape, Cluster Analysis, DNA, Drosophila Proteins, Drosophila melanogaster, HeLa Cells, Hemocytes, High-Throughput Screening Assays, Humans, Microfilament Proteins, Phenotype, RNA Interference, RNA Splicing, RNA, Double-Stranded, RNA, Small Interfering, SKP Cullin F-Box Protein Ligases, Tubulin, rho GTP-Binding Proteins
Although a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as a systematic method to define metazoan actin regulators based on visual phenotype. Using comparative screens in cultured Drosophila and human cells, we generated phenotypic profiles for annotated actin regulators together with proteins bearing predicted actin-binding domains. These phenotypic clusters for the known metazoan "actinome" were used to identify putative new core actin regulators, together with a number of genes with conserved but poorly studied roles in the regulation of the actin cytoskeleton, several of which we studied in detail. This work suggests that although our search for new components of the core actin machinery is nearing saturation, regulation at the level of nuclear actin export, RNA splicing, ubiquitination, and other upstream processes remains an important but unexplored frontier of actin biology.
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