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Publication Detail
A Pre-mRNA degradation pathway that selectively targets intron-containing genes requires the nuclear poly(A)-binding protein.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Lemieux C, Marguerat S, Lafontaine J, Barbezier N, Bähler J, Bachand F
  • Publication date:
    07/10/2011
  • Pagination:
    108, 119
  • Journal:
    Mol Cell
  • Volume:
    44
  • Issue:
    1
  • Status:
    Published
  • Country:
    United States
  • PII:
    S1097-2765(11)00678-2
  • Language:
    eng
  • Keywords:
    Cell Nucleus, Exoribonucleases, Exosome Multienzyme Ribonuclease Complex, Gene Expression Regulation, Gene Expression Regulation, Fungal, Introns, Models, Genetic, Poly A, Poly(A)-Binding Protein II, Poly(A)-Binding Proteins, Polyadenylation, RNA Precursors, RNA Splicing, Ribosomes, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Time Factors
Abstract
General discard pathways eliminate unprocessed and irregular pre-mRNAs to control the quality of gene expression. In contrast to such general pre-mRNA decay, we describe here a nuclear pre-mRNA degradation pathway that controls the expression of select intron-containing genes. We show that the fission yeast nuclear poly(A)-binding protein, Pab2, and the nuclear exosome subunit, Rrp6, are the main factors involved in this polyadenylation-dependent pre-mRNA degradation pathway. Transcriptome analysis and intron swapping experiments revealed that inefficient splicing is important to dictate susceptibility to Pab2-dependent pre-mRNA decay. We also show that negative splicing regulation can promote the poor splicing efficiency required for this pre-mRNA decay pathway, and in doing so, we identified a mechanism of cross-regulation between paralogous ribosomal proteins through nuclear pre-mRNA decay. Our findings unveil a layer of regulation in the nucleus in which the turnover of specific pre-mRNAs, besides the turnover of mature mRNAs, is used to control gene expression.
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