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Publication Detail
Biofunctionalized quantum dots for live monitoring of stem cells: applications in regenerative medicine.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    de Mel A, Oh J-T, Ramesh B, Seifalian AM
  • Publication date:
  • Pagination:
    335, 347
  • Journal:
    Regen Med
  • Volume:
  • Issue:
  • Status:
  • Country:
  • Language:
  • Keywords:
    AC133 Antigen, Antibodies, Antigens, CD, Biocompatible Materials, Cell Shape, Cell Survival, Coronary Artery Bypass, Endothelial Cells, Fluorescence, Glycoproteins, Hemolysis, Humans, Materials Testing, Microscopy, Confocal, Peptides, Quantum Dots, Regenerative Medicine, Spectroscopy, Fourier Transform Infrared, Staining and Labeling, Stem Cells, Thiomalates, Tissue Engineering
AIM: This study aimed to live monitor the degree of endothelial progenitor cell (EPC) integration onto tissue-engineering scaffolds by conjugating relevant antibodies to quantum dots (QDs). MATERIALS & METHODS: Biocompatible mercaptosuccinic acid-coated QDs were functionalized with two different antibodies to EPC (CD133 with QDs of 640 nm wavelength [λ] and later-stage mature EPCs; and von Willebrand factor with QDs of λ595 and λ555 nm) using conventional carbomide and N-hydroxysuccinimide chemistry. Biofunctionalization was characterized with Fourier-transform infrared spectroscopy. Cell viability assays and gross morphology observations confirmed cytocompatibility and normal patterns of celluar growth. The antigens corresponding to each state of cell maturation were determined using a single excitation at λ488 nm. RESULTS: The optimal concentrations of antibody-QD conjugates were biocompatible, hemocompatible and determined the state of EPC transformation to endothelial cells. CONCLUSION: Antibody-functionalized QDs suggest new applications in tissue engineering of polymer-based implants where cell integration can potentially be monitored without requiring the sacrifice of implants.
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