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Publication Detail
Establishment of a 3D engineered skeletal muscle-motoneuron co-culture using fibrin-cast gels
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  • Authors:
    Passey SL, Smith AST, Mudera V, Greensmith L, Baar K, Lewis MP
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  • Published proceedings:
    European Cells and Materials
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  • Name of conference:
    Tissue and Cell Engineering Society (TCES) meeting
  • Conference place:
    University of Leeds
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The successful in vitro engineering of complex tissues such as skeletal muscle would be of great benefit in the fields of tissue engineering and regenerative medicine, muscle physiology and neuromuscular research. A number of in vitro engineered models of skeletal muscle have been described that demonstrate many structural, biochemical and physiological similarities to in vivo muscle. As yet, however, there is no truly biomimetic in vitro model of skeletal muscle. An important aspect of in vivo muscle development and maintenance is the presence of a neuronal input via neuromuscular junctions (NMJs). Here we have increased the complexity of existing muscle models by introducing primary motoneurons with the aim of engineering a functional neuronal input in an established in vitro skeletal muscle model.
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