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Publication Detail
HtrA2 deficiency causes mitochondrial uncoupling through the F₁F₀-ATP synthase and consequent ATP depletion.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Plun-Favreau H, Burchell VS, Holmström KM, Yao Z, Deas E, Cain K, Fedele V, Moisoi N, Campanella M, Miguel Martins L, Wood NW, Gourine AV, Abramov AY
  • Publication date:
  • Pagination:
    e335, ?
  • Journal:
    Cell Death Dis
  • Volume:
  • Status:
    Published online
  • Country:
  • PII:
  • Language:
  • Keywords:
    Adenosine Triphosphate, Amino Acid Sequence, Animals, Cell Respiration, High-Temperature Requirement A Serine Peptidase 2, Membrane Potential, Mitochondrial, Mice, Mice, Knockout, Mitochondria, Mitochondrial Proteins, Molecular Sequence Data, Oxidative Phosphorylation, Proton-Translocating ATPases, Reactive Oxygen Species, Serine Endopeptidases
Loss of the mitochondrial protease HtrA2 (Omi) in mice leads to mitochondrial dysfunction, neurodegeneration and premature death, but the mechanism underlying this pathology remains unclear. Using primary cultures from wild-type and HtrA2-knockout mice, we find that HtrA2 deficiency significantly reduces mitochondrial membrane potential in a range of cell types. This depolarisation was found to result from mitochondrial uncoupling, as mitochondrial respiration was increased in HtrA2-deficient cells and respiratory control ratio was dramatically reduced. HtrA2-knockout cells exhibit increased proton translocation through the ATP synthase, in combination with decreased ATP production and truncation of the F1 α-subunit, suggesting the ATP synthase as the source of the proton leak. Uncoupling in the HtrA2-deficient mice is accompanied by altered breathing pattern and, on a cellular level, ATP depletion and vulnerability to chemical ischaemia. We propose that this vulnerability may ultimately cause the neurodegeneration observed in these mice.
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