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Publication Detail
Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Mason LJ, Lambrianides A, Haley JD, Manson JJ, Latchman DS, Isenberg DA, Rahman A
  • Publication date:
    10/06/2005
  • Pagination:
    R971, R983
  • Journal:
    Arthritis Research and Therapy
  • Volume:
    7
  • Issue:
    5
  • Print ISSN:
    1478-6362
  • Keywords:
    A, AND, Antibodies, Antibody, BINDING, cell, CELLS, Chromatin, COMPLEX, COMPLEXES, Condition, DNA, EXPRESSION, glomerulonephritis, Human, immunodeficiencies, immunodeficiency, IS, Lupus, MICE, MODEL, models, MURINE MODEL, OF, Ovary, PATHOGENESIS, pathogenicity, relationship, SEQUENCE, Severe Combined Immunodeficiency, SYSTEMIC, systemic lupus erythematosus, SYSTEMIC-LUPUS-ERYTHEMATOSUS, THE, VARIANT
  • Notes:
    WoS ID: 000231020200007 J
Abstract
When purified under rigorous conditions, some murine anti-double-stranded-DNA (anti-dsDNA) antibodies actually bind chromatin rather than dsDNA. This suggests that they may actually be antinucleosome antibodies that only appear to bind dsDNA when they are incompletely dissociated from nucleosomes. Experiments in murine models suggest that antibody - nucleosome complexes may play a crucial role in the pathogenesis of glomerulonephritis in systemic lupus erythematosus. Some human monoclonal anti- DNA antibodies are pathogenic when administered to mice with severe combined immunodeficiency (SCID). Our objective was to achieve stable expression of sequence-altered variants of one such antibody, B3, in Chinese hamster ovary (CHO) cells. Purified antibodies secreted by these cells were tested to investigate whether B3 is actually an antinucleosome antibody
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