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Publication Detail
Arginine transcriptional response does not require inositol phosphate synthesis.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Bosch D, Saiardi A
  • Publication date:
  • Pagination:
    38347, 38355
  • Journal:
    J Biol Chem
  • Volume:
  • Issue:
  • Country:
    United States
  • PII:
  • Language:
  • Keywords:
    Aldehyde Oxidoreductases, Arginine, Biocatalysis, Gene Expression Regulation, Fungal, Gene Knockout Techniques, Genetic Complementation Test, Humans, Inositol 1,4,5-Trisphosphate, Inositol Phosphates, Multienzyme Complexes, Mutation, Ornithine, Ornithine Carbamoyltransferase, Phosphotransferases (Alcohol Group Acceptor), Phosphotransferases (Carboxyl Group Acceptor), Promoter Regions, Genetic, Quaternary Ammonium Compounds, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Time Factors, Transcription, Genetic
Inositol phosphates are key signaling molecules affecting a large variety of cellular processes. Inositol-polyphosphate multikinase (IPMK) is a central component of the inositol phosphate biosynthetic routes, playing essential roles during development. IPMK phosphorylates inositol 1,4,5-trisphosphate to inositol tetrakisphosphate and subsequently to inositol pentakisphosphate and has also been described to function as a lipid kinase. Recently, a catalytically inactive mammalian IPMK was reported to be involved in nutrient signaling by way of mammalian target of rapamycin and AMP-activated protein kinase. In yeast, the IPMK homologue, Arg82, is the sole inositol-trisphosphate kinase. Arg82 has been extensively studied as part of the transcriptional complex regulating nitrogen sensing, in particular arginine metabolism. Whether this role requires Arg82 catalytic activity has long been a matter of contention. In this study, we developed a novel method for the real time study of promoter strength in vivo and used it to demonstrate that catalytically inactive Arg82 fully restored the arginine-dependent transcriptional response. We also showed that expression in yeast of catalytically active, but structurally very different, mammalian or plant IPMK homologue failed to restore arginine regulation. Our work indicates that inositol phosphates do not regulate arginine-dependent gene expression.
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