UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data shown on the profile page to:

http://www.ucl.ac.uk/finance/secure/research/post_award
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Optimization of chondrocyte isolation and characterization for large-scale cartilage tissue engineering.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Oseni AO, Butler PE, Seifalian AM
  • Publication date:
    01/05/2013
  • Pagination:
    41, 48
  • Journal:
    J Surg Res
  • Volume:
    181
  • Issue:
    1
  • Country:
    United States
  • PII:
    S0022-4804(12)00525-2
  • Language:
    eng
  • Keywords:
    Animals, Cell Count, Cell Separation, Chondrocytes, Collagenases, Fluorescent Antibody Technique, Humans, Male, Sheep, Tissue Engineering
Abstract
Advancements in cartilage tissue engineering have the potential to ameliorate facial and joint reconstructive surgery as we know it. The translation of in vitro models of cartilage regeneration into clinical scenarios is the next phase of cartilage tissue engineering research. To engineer larger, more robust, and clinical relevant constructs, a great number of viable chondrocytic cells are needed. However, there is a paucity of literature concerning the most favorable method of chondrocyte isolation. Isolation methods are inconsistent, resulting in small yields and poor cell quality, and thus unreliable neocartilage formation. This study aimed to optimize the chondrocyte isolation protocol to give a maximum yield with optimal cell viability for the engineering of large cartilaginous constructs such as the human nose and ear.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Authors
Research Department of General Surgery
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by