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Publication Detail
New developments in affinity chromatography with potential application in the production of biopharmaceuticals
  • Publication Type:
    Journal article
  • Publication Sub Type:
    JOUR
  • Authors:
    Lowe CR, Lowe AR, Gupta G
  • Publication date:
    2001
  • Pagination:
    561, 574
  • Journal:
    J Biochem Biophys Methods
  • Volume:
    49
  • Medium:
    1-3
  • Print ISSN:
    0165-022X
  • Keywords:
    Biopharmaceutics/ methods Chromatography, Affinity/ methods/trends Combinatorial Chemistry Techniques Crystallography, X-Ray/methods Drug Design Ligands Magnetic Resonance Spectroscopy
  • Notes:
    Lowe, C R Lowe, A R Gupta, G Review Journal of biochemical and biophysical methods S0165022X01002202 [pii] J Biochem Biophys Methods. 2001 Oct 30;49(1-3):561-74. Affinity chromatography is likely to play an increasingly important role in the purification of pharmaceutical proteins. This review describes new approaches to the design and synthesis of affinity ligands based on the ability to combine knowledge of X-ray crystallographic or NMR structures with defined or combinatorial chemical synthesis. The de novo design process is based on peptidal templates, complementarity to surface exposed residues and mimicking natural biological recognition. Examples of ligands designed to bind specifically to kallikrein, elastase, immunoglobulin G, insulin, alpha(1)-antitrypsin, clotting factor VII and glyco-proteins are given. The exceptional selectivity and stability of these synthetic ligands allows their use in harsh manufacturing environments.
Abstract
Affinity chromatography is likely to play an increasingly important role in the purification of pharmaceutical proteins. This review describes new approaches to the design and synthesis of affinity ligands based on the ability to combine knowledge of X-ray crystallographic or NMR structures with defined or combinatorial chemical synthesis. The de novo design process is based on peptidal templates, complementarity to surface exposed residues and mimicking natural biological recognition. Examples of ligands designed to bind specifically to kallikrein, elastase, immunoglobulin G, insulin, alpha(1)-antitrypsin, clotting factor VII and glyco-proteins are given. The exceptional selectivity and stability of these synthetic ligands allows their use in harsh manufacturing environments.
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