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Publication Detail
Improvements relating to fluorescence microscopy
  • Publication Type:
    Patent
  • Authors:
    Bain AJ
  • Publication date:
    17/01/2013
  • Application number:
    PCT/GB2012/051680
  • Patent number:
    WO2013008033-A1
  • Filed date:
    13/07/2012
  • Notes:
    http://www.google.com/patents/WO2013008033A1?cl=en
Abstract
Method of generating sub-wavelength resolution fluorescent images of a sample by linear combination of fluorescent images recorded in a series of time windows following pulsed excitation of fluorescent markers in the sample in the presence of stimulated emission depletion induced by a continuous wave depletion light source, said stimulated emission depletion resulting in a spatial variation in the observed fluorescence lifetime and an evolution in the effective point spread function (PSF) of the microscope with time..
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