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Publication Detail
Improvements relating to fluorescence microscopy
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    Bain AJ
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Method of generating sub-wavelength resolution fluorescent images of a sample by linear combination of fluorescent images recorded in a series of time windows following pulsed excitation of fluorescent markers in the sample in the presence of stimulated emission depletion induced by a continuous wave depletion light source, said stimulated emission depletion resulting in a spatial variation in the observed fluorescence lifetime and an evolution in the effective point spread function (PSF) of the microscope with time..
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