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Publication Detail
Adipose-derived stem cells inhibit the contractile myofibroblast in Dupuytren's disease
Abstract
BACKGROUND: In an attempt to provide minimally invasive treatment for Dupuytren's disease, percutaneous disruption of the affected tissue followed by lipografting is being trialled. Contractile myofibroblasts drive this fibroproliferative disorder whereas stem cells have recently been implicated in preventing fibrosis. Therefore, we tested the role of stem cells in modulating myofibroblast activity in Dupuytren's disease. METHODS: We compared the effect of co-culturing Dupuytren's myofibroblasts with either adipose or bone marrow-derived stem cells on isometric force contraction and associated levels of α-SMA mRNA and protein expression. We also tested the effect of these stem cells on Dupuytren's myofibroblast proliferation and assessed whether this was mediated by cell-cell contact or by a paracrine mechanism. RESULTS: Addition of adipose-derived stem cells to Dupuytren's myofibroblasts reduced the contraction of the latter with corresponding reduction of α-SMA protein expression, probably through a dilution effect. In contrast, bone marrow-derived stem cells increased the myofibroblast contractility. In addition, adipose-derived stem cells inhibit myofibroblast proliferation and mediate these effects by soluble factors, influenced by cell-cell contact dependent signalling. CONCLUSIONS: Adipose-derived stem cells inhibit the contractile myofibroblast in Dupuytren's disease and these findings lend support to the potential benefit of lipografting in conjunction with aponeurotomy as a novel strategy in the treatment of Dupuytren's disease.
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Inst of Orthopaedics & Musculosk Sci
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

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