UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at http://www.ucl.ac.uk/finance/research/post_award/post_award_contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Genome-wide analysis of poly(A) site selection in Schizosaccharomyces pombe.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Schlackow M, Marguerat S, Proudfoot NJ, Bähler J, Erban R, Gullerova M
  • Publication date:
    12/2013
  • Pagination:
    1617, 1631
  • Journal:
    RNA
  • Volume:
    19
  • Issue:
    12
  • Status:
    Published
  • Country:
    United States
  • PII:
    rna.040675.113
  • Language:
    eng
  • Keywords:
    RNA-Seq, fission yeast, genome-wide, polyadenylation, transcription termination, 3' Untranslated Regions, Base Sequence, Chromosome Mapping, Chromosomes, Fungal, Gene Expression Regulation, Fungal, Genes, Fungal, Genome, Fungal, Molecular Sequence Annotation, Molecular Sequence Data, Polyadenylation, RNA Cleavage, RNA, Fungal, RNA, Messenger, Schizosaccharomyces, Sequence Analysis, RNA, Transcription Termination, Genetic
Abstract
Polyadenylation of pre-mRNAs, a critical step in eukaryotic gene expression, is mediated by cis elements collectively called the polyadenylation signal. Genome-wide analysis of such polyadenylation signals was missing in fission yeast, even though it is an important model organism. We demonstrate that the canonical AATAAA motif is the most frequent and functional polyadenylation signal in Schizosaccharomyces pombe. Using analysis of RNA-Seq data sets from cells grown under various physiological conditions, we identify 3' UTRs for nearly 90% of the yeast genes. Heterogeneity of cleavage sites is common, as is alternative polyadenylation within and between conditions. We validated the computationally identified sequence elements likely to promote polyadenylation by functional assays, including qRT-PCR and 3'RACE analysis. The biological importance of the AATAAA motif is underlined by functional analysis of the genes containing it. Furthermore, it has been shown that convergent genes require trans elements, like cohesin for efficient transcription termination. Here we show that convergent genes lacking cohesin (on chromosome 2) are generally associated with longer overlapping mRNA transcripts. Our bioinformatic and experimental genome-wide results are summarized and can be accessed and customized in a user-friendly database Pomb(A).
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
Genetics, Evolution & Environment
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by