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Publication Detail
Diffusion microscopic MRI of the mouse embryo: Protocol and practical implementation in the splotch mouse model.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Norris FC, Siow BM, Cleary JO, Wells JA, De Castro SCP, Ordidge RJ, Greene NDE, Copp AJ, Scambler PJ, Alexander DC, Lythgoe MF
  • Publication date:
  • Pagination:
    731, 739
  • Journal:
    Magn Reson Med
  • Volume:
  • Issue:
  • Status:
  • Country:
    United States
  • Language:
  • Keywords:
    diffusion microscopic magnetic resonance imaging, mouse embryo, phenotyping, spina bifida, splotch mouse model, Animals, Diffusion Magnetic Resonance Imaging, Embryo, Mammalian, Image Enhancement, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, PAX3 Transcription Factor, Paired Box Transcription Factors, Prenatal Diagnosis, Reproducibility of Results, Sensitivity and Specificity, Specimen Handling, Spinal Cord
PURPOSE: Advanced methodologies for visualizing novel tissue contrast are essential for phenotyping the ever-increasing number of mutant mouse embryos being generated. Although diffusion microscopic MRI (μMRI) has been used to phenotype embryos, widespread routine use is limited by extended scanning times, and there is no established experimental procedure ensuring optimal data acquisition. METHODS: We developed two protocols for designing experimental procedures for diffusion μMRI of mouse embryos, which take into account the effect of embryo preparation and pulse sequence parameters on resulting data. We applied our protocols to an investigation of the splotch mouse model as an example implementation. RESULTS: The protocols provide DTI data in 24 min per direction at 75 μm isotropic using a three-dimensional fast spin-echo sequence, enabling preliminary imaging in 3 h (6 directions plus one unweighted measurement), or detailed imaging in 9 h (42 directions plus six unweighted measurements). Application to the splotch model enabled assessment of spinal cord pathology. CONCLUSION: We present guidelines for designing diffusion μMRI experiments, which may be adapted for different studies and research facilities. As they are suitable for routine use and may be readily implemented, we hope they will be adopted by the phenotyping community.
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