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Publication Detail
Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Research Support, Non-U.S. Gov't
  • Authors:
    Huy TQ, Van Chung P, Thuy NT, Blanco-Andujar C, Thanh NTK
  • Publication date:
  • Pagination:
    73, 82
  • Journal:
    Faraday discussions
  • Volume:
  • Medium:
  • Status:
  • Print ISSN:
  • Language:
  • Keywords:
    Vibrio cholerae, Ferric Compounds, Staphylococcal Protein A, Bacteriological Techniques, Water Microbiology, Particle Size, Surface Properties, Nanoparticles
  • Addresses:
    National Institute of Hygiene and Epidemiology, 1 Yersin Street, Hai Ba Trung District, Hanoi, Vietnam.
Pathogen separation is of great significance for precise detection and prevention of disease outbreaks. For the first time, protein A conjugated with chitosan-coated iron oxide nanoparticles was prepared for pathogen separation at low concentrations from liquid samples. Vibrio cholerae O1 (VO1) bacteria were used for testing the effectiveness of this conjugate. Transmission electron microscopy (TEM) was used to confirm the presence of captured VO1. The results showed that, after binding with a specific antibody, the conjugate allows separation of VO1 bacteria from water samples at a concentration as low as 10 cfu mL(-1). Moreover, the conjugate can be used in parallel with conventional or modern diagnostic tests for quick and accurate detection of pathogens.
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