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Publication Detail
Exposure of Porphyromonas gingivalis to red light in the presence of the light-activated antimicrobial agent toluidine blue decreases membrane fluidity
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Publication Type:Journal article
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Publication Sub Type:Article
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Authors:Bhatti M, MacRobert A, Henderson B, Wilson M
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Publication date:08/2002
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Pagination:118, 122
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Journal:Current Microbiology
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Volume:45
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Issue:2
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Print ISSN:0343-8651
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Keywords:AGENT, Antimicrobial, EXPOSURE, Gingivalis, LIGHT, membrane, Micro, MS, Other, Porphyromonas gingivalis, TOLUIDINE BLUE, Antimicrobial, Membrane, ADAPTATION, AGENTS, ALPHA-TOCOPHEROL, BACILLUS-SUBTILIS, BACTERIA, CELLS, FLUORESCENCE, LETHAL PHOTOSENSITIZATION, LIPID-PEROXIDATION, MECHANISM, PARAMETERS, PHOTOSENSITIZATION, PORPHYROMONAS-GINGIVALIS, PROTEINS, TEMPERATURE
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Addresses:UCL, Eastman Dent Inst Oral Hlth Care Sci, Dept Microbiol, London WC1X 8LD, EnglandRoyal Free & Univ Coll Med Sch, Natl Med Laser Ctr, London W1W 7EJ, EnglandUCL, Eastman Dent Inst, Cellular Microbiol Res Grp, London WC1X 8LD, England
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Notes:WoS ID: 000176581500008 Times Cited: 4ArticleEnglishWilson, MUCL, Eastman Dent Inst Oral Hlth Care Sci, Dept Microbiol, 256 Grays Inn Rd, London WC1X 8LD, EnglandCited References Count: 17569CBSPRINGER-VERLAG175 FIFTH AVE, NEW YORK, NY 10010 USANEW YORK
Abstract
Porphyromonas gingivalis, one of the etiological agents of periodontitis, can be killed by red light in the presence of toluidine blue. The purpose of this study was to deter-mine whether this light-induced killing was accompanied by changes in the fluidity of the organism's cytoplasmic membrane. A suspension of the organism was exposed to red light in the presence of toluidine blue, and the membrane fluidity was monitored spectrofluorimetrically by using the membrane probe trimethylammonium diphenyl hexatriene. The fluidity of the organism's cytoplasmic membrane was found to decrease significantly during lethal photosensitization, and this was accompanied by membrane condensation and vacuolation of the cells. Although changes in membrane fluidity are often attributable to lipid peroxidation, malonaldehyde (a product of lipid peroxidation) was not detectable. The disruption of membrane functions associated with a decreased membrane fluidity may contribute to the bactericidal effect of light-activated toluidine blue
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