Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to
your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:
Email: portico-services@ucl.ac.uk
Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Email: portico-services@ucl.ac.uk
Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Inorganic polyphosphate and energy metabolism in mammalian cells.
-
Publication Type:Journal article
-
Publication Sub Type:Journal Article
-
Authors:Pavlov E, Aschar-Sobbi R, Campanella M, Turner RJ, Gómez-García MR, Abramov AY
-
Publication date:26/03/2010
-
Pagination:9420, 9428
-
Journal:J Biol Chem
-
Volume:285
-
Issue:13
-
Status:Published
-
Country:United States
-
PII:S0021-9258(19)54998-5
-
Language:eng
-
Keywords:Adenosine Triphosphate, Animals, Electrophoresis, Energy Metabolism, Fluorescent Dyes, Hydrolysis, Membrane Potentials, Mitochondria, Oligomycins, Oxidative Phosphorylation, Oxygen Consumption, Polymers, Polyphosphates, Rats, Rats, Sprague-Dawley
-
Author URL:
Abstract
Inorganic polyphosphate (poly P) is a polymer made from as few as 10 to several hundred phosphate molecules linked by phosphoanhydride bonds similar to ATP. Poly P is ubiquitous in all mammalian organisms, where it plays multiple physiological roles. The metabolism of poly P in mammalian organisms is not well understood. We have examined the mechanism of poly P production and the role of this polymer in cell energy metabolism. Poly P levels in mitochondria and intact cells were estimated using a fluorescent molecular probe, 4',6-diamidino-2-phenylindole. Poly P levels were dependent on the metabolic state of the mitochondria. Poly P levels were increased by substrates of respiration and in turn reduced by mitochondrial inhibitor (rotenone) or an uncoupler (carbonyl cyanide p-trifluoromethoxyphenylhydrazone). Oligomycin, an inhibitor of mitochondrial ATP-synthase, blocked the production of poly P. Enzymatic depletion of poly P from cells significantly altered the rate of ATP metabolism. We propose the existence of a feedback mechanism where poly P production and cell energy metabolism regulate each other.
› More search options
UCL Researchers