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Publication Detail
Monitoring collagen gelling by elastic scattering spectroscopy (ESS)
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  • Authors:
    Jawad H, Austwick M, MacRobert S, Kuraishi A, Tully F, Alkseeva T, Brown R
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    European Cells and Materials
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  • Name of conference:
    Tissue and Cell Engineering Society (TCES) meeting
  • Conference place:
    University of Manchester
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Introduction Collagen is being used extensively in tissue engineering and on a larger scale in the field of cosmetic surgery. It is either used as a gel or plastically compressed sheet1. The fundamental science behind collagen gelling has been studied but little is known about the precise timing of gelling and the variables that affect gelling in the first 30 minutes. Critically, before collagen can be engineered as a predictable functional material we must be able to control fibril aggregation and gel formation. Here we report on the use of elastic scattering spectroscopy (ESS) to detect changes in scattering in rat tail and GMP bovine skin collagen during gelling. Effect of cell seeding on gelling is also reported.
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