UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Melatonin protects from, but does not reverse, the effects of mediators of sepsis on liver bioenergetics
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Basile M, Romeo C, Gitto E, Spitz L, Pierro A, Eaton S
  • Publication date:
    2004
  • Pagination:
    263, 266
  • Journal:
    Pediatric Surgery International
  • Volume:
    20
  • Issue:
    4
  • Print ISSN:
    0179-0358
  • Keywords:
    1, 10, 1115, 2, A, AND, bioenergetics, cell, CELLS, CONSUMPTION, DAMAGE, DYSFUNCTION, ENERGY, Energy Metabolism, Energy-metabolism, FOR, free radical, FUNCTION, H2O2, HAD, hepatocyte, hepatocyte oxygen consumption, hepatocytes, Hydrogen, hydrogen peroxide, HYDROGEN-PEROXIDE, IS, LIVER, Low, MEDIATOR, melatonin, METABOLISM, METHODS, Mitochondrial, MITOCHONDRIAL FUNCTION, NEONATAL, NEONATE, neonates, OF, old, OXIDATION, oxidative, oxidative metabolism, Oxidative Stress, OXIDATIVE-METABOLISM, OXYGEN, Oxygen Consumption, Oxygen-consumption, Palmitate, PATHOGENESIS, peroxide, PROTECTION, PROTECTS, RAT, RATS, Reactive oxygen, reactive oxygen species, REDUCE, Result, RISK, ROS, sepsis, stress, SUBSEQUENT, Suckling rat, Suckling rats, THE, TIME
Abstract
Background. Reactive oxygen species (ROS) have been reported to play a significant role in the pathogenesis of sepsis and liver dysfunction. In particular, neonates are at risk for sepsis and have less protection against oxidation. Melatonin has been reported to reduce the oxidative stress status in neonates with sepsis. Little is known about the effect of melatonin on liver bioenergetics. The aim of this study was to investigate the protective effect of melatonin on hepatocyte oxidative energy metabolism against hydrogen peroxide (H2O2), a free radical mediator of septic damage. Methods. Hepatocytes were isolated from neonatal suckling rats (11-15 days old). The cells, respiring on palmitate, were exposed to H2O2 at the concentration of 2 mmol/l, melatonin alone at 1 mumol/l or 10 mumol/l, or H2O2 plus melatonin at each of the two concentrations. Oxygen consumption was measured polarographically. In subsequent experiments, melatonin was added after the hydrogen peroxide. Results. Hydrogen peroxide significantly reduced hepatocyte oxygen consumption (p<0.001), but melatonin added at the same time was able to prevent this effect (p<0.001). However, melatonin at a low dose significantly inhibited hepatocyte oxygen consumption (p<0.001), an effect which has not been previously described. When melatonin was added to cells after they had been exposed to hydrogen peroxide, a beneficial effect was not observed, indicating that melatonin is not able to reverse the effects of hydrogen peroxide. Conclusion. Melatonin has a protective effect on hepatocyte oxidative metabolism, improving mitochondrial function by counteracting oxidative stress
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
ICH Development Bio & Cancer Prog
Author
UCL GOS Institute of Child Health
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by