The volumetric oxygen transfer coefficient (kLa) in shaken microplate microbial cultivations has been modelled using dimensionless groups. The kLa correlation was derived from experimental measurements of air-liquid specific surface area and kLa values for three microwell geometries operated over a range of shaking frequencies and diameters. The air-liquid surface area was determined from the rate of evaporation and also from high-speed video photography. kLa values were calculated from the mass transfer limited growth rate of a strict aerobe, Bacillus subtilis, and were also directly measured using the dynamic gassing out technique. For both surface area and kLa measurements there was good agreement between each of the two methods used. The overall correlation for kLa values comprised two separate correlations based on different dimensionless groups. The first described the increase in specific air-liquid surface area (af/ai) as a function of Froude (Fr) and Bond (Bo) numbers and is represented as: af/ai = c1.Frc2.Boc3 where c1 is a geometry dependent constant, c2 ranged from 0.51 to 0.86 and c3 ranged from 0.03 to 0.18. As expected, when the diameter of a microwell decreases, the significance of surface forces increases and the importance of inertial forces decrease. The second correlation related the Sherwood (Sh) number in terms of Reynolds (Re) and Schmidt (Sc) number and is represented as Sh = 0.19.Re0.68.Sc0.36. This correlation was independent of microwell geometry. By combining the two correlations it was possible to predict kLa values over a wide range of operating conditions. These models provide a rigorous engineering basis for more reproducible and rational selection of operating conditions for microplate scale cell cultivation.